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Publicación del Grupo de Investigación BIT en Science

La  Publicación en la revista Science (de la que se hacen eco el periódico el Mundo y la U.P.M.) del objetivo alcanzado por un consorcio franco-español formado por el Centro Nacional de Investigación Científica francés CNRS, la École Polytechnique de Paris y el grupo Biomedical Image Technologies, dirigido por el catedrático Andrés Santos, de la Universidad Politécnica de Madrid y miembro del Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN).

Cell Lineage Reconstruction of Early Zebrafish Embryos Using Label-Free Nonlinear Microscopy

Nicolas Olivier,1,* Miguel A. Luengo-Oroz,2,* Louise Duloquin,3,* Emmanuel Faure,4 Thierry Savy,4 Israël Veilleux,1 Xavier Solinas,1 Delphine Débarre,1 Paul Bourgine,4,5 Andrés Santos,2 Nadine Peyriéras,3,6, Emmanuel Beaurepaire1,


Quantifying cell behaviors in animal early embryogenesis remains a challenging issue requiring in toto imaging and automated image analysis. We designed a framework for imaging and reconstructing unstained whole zebrafish embryos for their first 10 cell division cycles and report measurements along the cell lineage with micrometer spatial resolution and minute temporal accuracy. Point-scanning multiphoton excitation optimized to preferentially probe the innermost regions of the embryo provided intrinsic signals highlighting all mitotic spindles and cell boundaries. Automated image analysis revealed the phenomenology of cell proliferation. Blastomeres continuously drift out of synchrony. After the 32-cell stage, the cell cycle lengthens according to cell radial position, leading to apparent division waves. Progressive amplification of this process is the rule, contrasting with classical descriptions of abrupt changes in the system dynamics.


1 Laboratory for Optics and Biosciences, Ecole Polytechnique, CNRS, INSERM, Palaiseau, France.
2 Biomedical Image Technologies, Universidad Politécnica de Madrid, and Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), Madrid, Spain.
3 Neurobiologie et Développement, Institut de Neurobiologie Alfred Fessard, CNRS, Gif/Yvette, France.
4 Centre de Recherche en Epistémologie Appliquée, Ecole Polytechnique, CNRS, Paris, France.
5 Réseau National des Systèmes Complexes, 57-59 rue Lhomond, Paris, France.
6 Institut des Systèmes Complexes Paris Ile-de-France, 57-59 rue Lhomond, Paris, France.

* These authors contributed equally to this work.